Composite

Part:BBa_K1162013:Design

Designed by: Kathleen Miller   Group: iGEM13_Utah_State   (2013-09-15)


promoter+rbs+N terminal His Tag and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 269
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 788
    Illegal XhoI site found at 689
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RFC 23 when making the N terminal His Tag and GFP fusion.


Source

E. coli.

References